Apoptosis of human glioma cells in vitro and in vivo induced by a neutralizing antibody against human basic fibroblast growth factor

Nozomu Murai, Tetsuya Ueba, Jun A. Takahashi, Hong Qiong Yang, Haruhiko Kikuchi, Hiroshi Hiai, Masakazu Hatanaka, Manabu Fukumoto

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Basic fibroblast growth factor (bFGF) is mitogenic to neuroectoderm- and mesoderm-derived cells and is a potent angiogenic factor. Abundant amounts of this factor and its receptor are detected in human glioma tissues and cells, and bFGF in glioma is thought to be involved in autonomous cell growth as an autocrine growth factor. A neutralizing mouse monoclonal antibody (MAb) against bFGF, 3H3 MAb, has been shown to inhibit both in vitro and in vivo growth of human glioma cell lines. This study shows that the human glioma cell lines U-87MG and U-251MG, which express high levels of bFGF and its receptor, can be induced to undergo apoptosis when cultured with 3H3 MAb. It is also demonstrated that 3H3 MAb can cause apoptosis in the same glioma cells that were transplanted into nude mice. Furthermore, enforced overexpression of bcl-2 protein by gene transfection prevented 3H3 MAb- induced apoptosis of glioma cells. It is concluded that induction of apoptosis by the neutralizing antibody is a promising therapeutic strategy for glioma.

Original languageEnglish
Pages (from-to)1072-1077
Number of pages6
JournalJournal of neurosurgery
Volume85
Issue number6
DOIs
Publication statusPublished - 1996 Jan 1

Keywords

  • anti-basic fibroblast growth factor antibody
  • apoptosis
  • human glioma
  • immunotherapy

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

Fingerprint Dive into the research topics of 'Apoptosis of human glioma cells in vitro and in vivo induced by a neutralizing antibody against human basic fibroblast growth factor'. Together they form a unique fingerprint.

  • Cite this

    Murai, N., Ueba, T., Takahashi, J. A., Yang, H. Q., Kikuchi, H., Hiai, H., Hatanaka, M., & Fukumoto, M. (1996). Apoptosis of human glioma cells in vitro and in vivo induced by a neutralizing antibody against human basic fibroblast growth factor. Journal of neurosurgery, 85(6), 1072-1077. https://doi.org/10.3171/jns.1996.85.6.1072