Apoptosis occurs in granulosa cells but not cumulus cells in the atretic graafian follicles in multiparous pig ovaries

Noboru Manabe, Yuzuru Imai, Akira Myoumoto, Yoshihiro Kimura, Miki Sugimoto, Yoshinori Okamura, Manabu Fukumoto, Kazuhiro Sakamaki, Hajime Miyamoto

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

The process of apoptosis in granulosa and cumulus cells of ovarian follicles during follicular atresia was investigated in multiparous pigs. In healthy follicles, no apoptotic cells were observed histochemically, and no DNA ladder formation or Ca2+/Mg2+-dependent endonuclease activity were detected biochemically in granulosa or cumulus cells. In the follicles in the early stage of atresia, apoptosis occurred in granulosa cells located on the inner surface of the follicular wall but not cumulus cells. DNA ladder formation was observed in granulosa but not cumulus cells, and high levels of endonuclease activity were demonstrated only in the granulosa cells. No endonuclease activity was detected in cumulus cells obtained from the same atretic follicles. These findings suggest a lack of apoptotic cell death in cumulus cells in atretic follicles in multiparous pig ovaries.

Original languageEnglish
Pages (from-to)85-92
Number of pages8
JournalACTA HISTOCHEMICA ET CYTOCHEMICA
Volume30
Issue number1
DOIs
Publication statusPublished - 1997

Keywords

  • Apoptosis
  • Cumulus cell
  • Endonuclease
  • Granulosa cell
  • Multiparous pig ovary

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology

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