Apoptosis induction by aluminum phthalocyanine tetrasulfonate-based sonodynamic therapy in HL-60 cells

Yumiko Iwase, Nagahiko Yumita, Koji Nishi, Hiroyuki Kuwahara, Toshio Fukai, Toshihiko Ikeda, Fu Shih Chen, Yasunori Momose, Shin Ichiro Umemura

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5 Citations (Scopus)


The present study aims to investigate sonodynamically-induced apoptosis using the phthalocyanine, chloroaluminum phthalocyanine tetrasulfonate (AlPcTS). HL-60 cells were exposed to ultrasound for up to 3 min in the absence and presence of AlPcTS. Apoptosis was analyzed by cell morphology, DNA fragmentation, and caspase-3 activity. Electron spin resonance was used to measure reactive oxygen species. The number of apoptotic cells showing membrane blebbing and cell shrinkage after combined treatment (ultrasound and AlPcTS) was significantly higher than following other treatments, including ultrasound alone and AlPcTS alone. Furthermore, DNA ladder formation, caspase-3 activation and enhanced nitroxide generation were observed in cells treated with ultrasound and AlPcTS. Sonodynamically induced apoptosis, caspase-3 activation, and nitroxide generation were significantly suppressed by histidine. The significant reduction by histidine indicated that ultrasonically generated reactive oxygen species, such as singlet oxygen, is an important mediator of sonodynamically-induced apoptosis.

Original languageEnglish
Article number07HD05
JournalJapanese journal of applied physics
Issue number7
Publication statusPublished - 2015 Jul 1

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)


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