Apoptosis induced by the sonomechanical effects of low intensity pulsed ultrasound in a human leukemia cell line

Loreto B. Feril, Takashi Kondo, Zheng Guo Cui, Yoshiaki Tabuchi, Qing Li Zhao, Hidetaka Ando, Takuro Misaki, Hideki Yoshikawa, Shin Ichiro Umemura

Research output: Contribution to journalArticlepeer-review

120 Citations (Scopus)

Abstract

To obtain an optimal condition for ultrasound (US)-induced apoptosis that could be useful for cancer therapy, we applied low intensity pulsed US to sonicate U937 cells in vitro. Cells were then incubated at different time intervals before measuring apoptosis. The apoptosis was assessed by DNA fragmentation and phosphatidylserine externalization. The pattern of the decrease in mitochondrial membrane potential was determined by flow cytometry. Optimal apoptosis (70.0±13.8%) with minimal lysis was attained with 1 MHz ultrasound 0.3 W/cm2, 10% duty factor at 100 Hz for 1 min) at 12 h after sonication. Lack of US-induced free radical detection and absence of Heme oxygenase-1, an intracellular oxidative stress marker, up-regulation in cells, suggest that sonomechanical, not sonochemical, effects are the main mechanism involved.

Original languageEnglish
Pages (from-to)145-152
Number of pages8
JournalCancer Letters
Volume221
Issue number2
DOIs
Publication statusPublished - 2005 Apr 28

Keywords

  • Apoptosis
  • Low intensity ultrasound
  • Sonomechanical effects

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Apoptosis induced by the sonomechanical effects of low intensity pulsed ultrasound in a human leukemia cell line'. Together they form a unique fingerprint.

Cite this