Apoptosis in HepG2 cells induced by Pteris semipinnata extract 5F involves p53 activation and VEGF inhibition

Li Li, Ying Nian Lü, Yi Liu, Ke Feng Wu, George G. Chen, Nian Ci Liang

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Objective: To observe the effect of Pteris semipinnata extract, ent-11α-hydroxy-15-oxokaur-16-en-19-oic-acid (5F), on human hepatoma HepG2 cells about the proliferation and to investigate the role of p53, VEGF, and Caspase-3 in 5F-induced HepG2 cell apoptosis. Methods: MTT Assay was used to determine the effect of 5F on proliferation of HepG2 cells, and cytoplasmic mono- and oligonucleosomes of HepG2 cells treated with 5F was assessed with Cell Death Detection ELISA Kit for determining whether 5F can induce apoptosis. Apoptotic nuclear morphology was assessed using Hoechst/PI assay. The effect on the expression levels of p53 and VEGF protein in HepG2 cells was detected by Western blotting analysis , and Caspase-3 activity was measured by a Caspase-3 Colorimetric Assay Kit. Results: The cytotoxicity of 5F on HepG2 cells was elevated with 5F concentration increasing. Cytoplasmic mono- and oligonucleosomes were induced by 5F in HepG2 cells, and the induction was in a concentration-dependent manner. After treatment with 5F, the apoptotic change such as chromatin condensation was confirmed by Hoechst/ PI staining. The level of p53 in nucleus was significantly elevated in HepG2 cells after 5F treatment, but the level of VEGF in cytosol was decreased. Moreover, the activity of Caspase-3 increased in a concentration-dependent manner. Conclusion: 5F mediated apoptosis involves p53 and Caspase-3 activation, VEGF down regulation in HepG2 cells. 5F might have a therapeutic value against human cancer cell lines and especially on hepatocellular carcinoma (HCC) cells.

Original languageEnglish
Pages (from-to)241-245
Number of pages5
JournalChinese Traditional and Herbal Drugs
Volume41
Issue number2
Publication statusPublished - 2010 Feb 1

Keywords

  • Apoptosis
  • Hepatocellular carcinoma (HCC)
  • Pteris semipinnata L.
  • ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid (5F)

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Pharmacology (medical)

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