Apolipoprotein E stimulates bone formation on titanium in vitro

Takashi Kodama, Tetsuya Goto, Toru Ishibe, Shigeru Kobayashi, Tetsu Takahashi

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Objective: To examine the effects of apolipoprotein E2, a strongly expressed molecule during titanium implant healing, on bone formation by rat primary osteoblasts over titanium. Materials and Methods: Primary rat osteoblasts were cultured on titanium in the presence of 0.01-1.0 μg/mL apolipoprotein E2. After 2 or 3 weeks of culture, bone formation was analysed by staining the bone-like nodules with calcein blue. The mRNAs of the osteogenic genes type I collagen, osteopontin, and osteocalcin, were analysed using semi-quantitative reverse transcriptase-polymerase chain reaction. Results: Apolipoprotein E2 enhanced bone-like nodule formation on titanium in a dose-dependent manner. Apolipoprotein E2 strongly upregulated osteopontin and osteocalcin mRNA expression, but had only a small effect on type I collagen mRNA expression. Conclusion: Apolipoprotein E2 stimulated bone formation on titanium disks in vitro. This may lead to a new approach to accelerating implant healing.

Original languageEnglish
Pages (from-to)96-100
Number of pages5
JournalAsian Journal of Oral and Maxillofacial Surgery
Issue number2
Publication statusPublished - 2007 Jan 1
Externally publishedYes


  • Apolipoproteins
  • Bone and bones
  • Osteoblasts
  • Osteocalcin
  • Titanium

ASJC Scopus subject areas

  • Surgery
  • Oral Surgery
  • Orthopedics and Sports Medicine

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