Apg10p, a novel protein-conjugating enzyme essential for autophagy in yeast

Takahiro Shintani, Noboru Mizushima, Yoko Ogawa, Akira Matsuura, Takeshi Noda, Yoshinori Ohsumi

Research output: Contribution to journalArticlepeer-review

222 Citations (Scopus)

Abstract

Autophagy is a cellular process for bulk degradation of cytoplasmic components. The attachment of Apg12p, a modifier with no significant similarity to ubiquitin, to Apg5p is crucial for autophagy in yeast. This reaction proceeds in a ubiquitination-like manner, and requires Apg7p and Apg10p. Apg7p exhibits a considerable similarity to ubiquitin-activating enzyme (E1) and is found to activate Apg12p with ATP hydrolysis. Apg10p, on the other hand, shows no significant similarity to other proteins whose functions are known. Here, we show that after activation by Apg7p, Apg12p is transferred to the Cys-133 residue of Apg10p to form an Apg12p-Apg10p thioester. Cells expressing Apg10p(C133S) do not generate the Apg12p-Apg5p conjugate, which leads to defects in autophagy and cytoplasmto-vacuole targeting of aminopeptidase I. These findings indicate that Apg10p is a new type of protein-conjugating enzyme that functions in the Apg12p-Apg5p conjugation pathway.

Original languageEnglish
Pages (from-to)5234-5241
Number of pages8
JournalEMBO Journal
Volume18
Issue number19
DOIs
Publication statusPublished - 1999 Oct 1
Externally publishedYes

Keywords

  • Autophagy
  • Protein conjugating enzyme
  • Protein conjugation
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint

Dive into the research topics of 'Apg10p, a novel protein-conjugating enzyme essential for autophagy in yeast'. Together they form a unique fingerprint.

Cite this