Antisense RNA of the latent period gene (MER5) inhibits the differentiation of murine erythroleukemia cells

Yasuhisa Nemoto, Tohru Yamamoto, Shinji Takada, Yasuhisa Matsui, Masuo Obinata

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The MER5 cDNA was cloned from RNA preferentially synthesized in murine erythroleukemia (MEL) cells during the early period of MEL cell differentiation. To understand the role of the MER5 gene in the differentiation, we have transferred the MER5 cDNA into MEL cells in both sense and antisense orientations under control of the promoter of the human metallothionein gene. Only in the transformants with the antisense MER5 cDNA, did their elevated expression inhibit differentiation. The results suggests that the MER5 gene product may promote early events in the differentiation of MEL cells.

Original languageEnglish
Pages (from-to)261-265
Number of pages5
JournalGene
Volume91
Issue number2
DOIs
Publication statusPublished - 1990 Jul 16

Keywords

  • DNA transfection
  • Recombinant DNA
  • erythroid differentiation

ASJC Scopus subject areas

  • Genetics

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