Antioxidant capacity of BO-653, 2,3-dihydro-5-hydroxy-4,6-di-tert-butyl-2,2-dipentylbenzofuran, and uric acid as evaluated by ORAC method and inhibition of lipid peroxidation

Etsuo Niki, Akiko Fukuhara, Yo Omata, Yoshiro Saito, Yasukazu Yoshida

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The role of radical-scavenging antioxidant against oxidative stress has received much attention. The antioxidant capacity has been assessed by various methods. Above all, oxygen radical absorbance capacity (ORAC) has been frequently employed [Prior et.al., J. Agric. Food Chem. 2005, 53, 4290]. In the present study, the antioxidant capacity of 2,3-dihydro-5-hydroxy-4,6-di-tert-butyl-2,2-dipentylbenzofuran (BO-653) and uric acid was assessed by ORAC method using pyranine as a reference probe and compared with that against lipid peroxidation of human plasma. It was found that BO-653 was assessed to be much less potent than uric acid by ORAC method, whereas BO-653 exerted much higher antioxidant activity than uric acid against plasma lipid peroxidation. The reason for such discrepancy is discussed. The results suggest that ORAC method is suitable for the assessment of free radical scavenging capacity, but not for the assessment of antioxidant capacity against lipid peroxidation in plasma.

Original languageEnglish
Pages (from-to)2464-2466
Number of pages3
JournalBioorganic and Medicinal Chemistry Letters
Volume18
Issue number7
DOIs
Publication statusPublished - 2008 Apr 1
Externally publishedYes

Keywords

  • AAPH
  • Antioxidant capacity
  • BO-653
  • Lipid peroxidation
  • ORAC
  • Pyranine
  • Uric acid

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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