Antinociceptive effect of different types of calcium channel inhibitors and the distribution of various calcium channel α 1 subunits in the dorsal horn of spinal cord in mice

Manabu Murakami, Osamu Nakagawasai, Takashi Suzuki, Izadi I. Mobarakeh, Yumiko Sakurada, Astunobu Murata, Fumihiro Yamadera, Ichiro Miyoshi, Kazuhiko Yanai, Koichi Tan-No, Hironobu Sasano, Takeshi Tadano, Toshihiko Iijima

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

To understand better which voltage-dependent calcium channels (VGCCs) are involved in nociceptive neurotransmission, we investigated the pharmacological properties and distribution of VGCCs in the mouse spinal cord. A behavioral assay revealed that intrathecal injections of ω-agatoxin TK, ω-agatoxin IVA, ω-conotoxin GVIA, and SNX-482, which block P/Q-, P/Q-, N-, and R-type calcium channels, respectively, produced analgesic effects, while an L-type channel blocker had no such effect. An electrophysiological study demonstrated the presence of various types of VGCCs within dorsal root ganglion (DRG) neurons. Immunohistochemistry revealed distinct localization of P/Q-, N-, L-, and R-type calcium channel subunits to the dorsal horn of the spinal cord. The results of this study revealed the localization and functions of several calcium channels that are involved in nociceptive neurotransmission within the dorsal horn of the mouse spinal cord.

Original languageEnglish
Pages (from-to)122-129
Number of pages8
JournalBrain research
Volume1024
Issue number1-2
DOIs
Publication statusPublished - 2004 Oct 22

Keywords

  • Calcium channel
  • Mouse
  • Nociception
  • SNX-482
  • Toxin
  • ω-Agatoxin TK

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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