TY - JOUR
T1 - Antimicrobial susceptibilities of Clostridium difficile isolated in Japan
AU - Kunishima, Hiroyuki
AU - Chiba, Junichi
AU - Saito, Michiko
AU - Honda, Yoshihiro
AU - Kaku, Mitsuo
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2013/4
Y1 - 2013/4
N2 - Clostridium difficile is a common causative organism of antimicrobial-associated diarrhea and is often responsible for nosocomial infection. C. difficile infection has traditionally been treated with metronidazole (MNZ) or vancomycin (VCM); however, MNZ-resistant strains have reported in some countries. In this study the broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) of 15 drugs against 157 clinical isolates of C. difficile in Japan. All C. difficile isolates tested were susceptible to MNZ; MIC50 was 0.25 μg/ml, MIC90 was 0.5 μg/ml, and MIC range was 0.06-1 μg/ml. C. difficile isolates were also susceptible to VCM; MIC50 was 0.5 μg/ml, MIC90 was 1 μg/ml, and MIC range was 0.12-2 μg/ml. Susceptibility to teicoplanin was also similar: MIC50 was 0.12 μg/ml, MIC90 was 0.12 μg/ml, and MIC range was 0.03-0.25 μg/ml. The susceptibility of C. difficile isolates must be monitored continuously because a strain with reduced susceptibility to MNZ or VCM might emerge in the future.
AB - Clostridium difficile is a common causative organism of antimicrobial-associated diarrhea and is often responsible for nosocomial infection. C. difficile infection has traditionally been treated with metronidazole (MNZ) or vancomycin (VCM); however, MNZ-resistant strains have reported in some countries. In this study the broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) of 15 drugs against 157 clinical isolates of C. difficile in Japan. All C. difficile isolates tested were susceptible to MNZ; MIC50 was 0.25 μg/ml, MIC90 was 0.5 μg/ml, and MIC range was 0.06-1 μg/ml. C. difficile isolates were also susceptible to VCM; MIC50 was 0.5 μg/ml, MIC90 was 1 μg/ml, and MIC range was 0.12-2 μg/ml. Susceptibility to teicoplanin was also similar: MIC50 was 0.12 μg/ml, MIC90 was 0.12 μg/ml, and MIC range was 0.03-0.25 μg/ml. The susceptibility of C. difficile isolates must be monitored continuously because a strain with reduced susceptibility to MNZ or VCM might emerge in the future.
KW - Antibiotic susceptibility
KW - Clostridium difficile
KW - Japan
KW - Metronidazole
KW - Vancomycin
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U2 - 10.1007/s10156-013-0553-5
DO - 10.1007/s10156-013-0553-5
M3 - Article
C2 - 23338015
AN - SCOPUS:84880700886
VL - 19
SP - 360
EP - 362
JO - Journal of Infection and Chemotherapy
JF - Journal of Infection and Chemotherapy
SN - 1341-321X
IS - 2
ER -