Antigenic sites of the muscle protein, paramyosin, in Caenorhabditis elegans determined with exon-expression plasmid.

H. Kagawa, K. Gengyo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Sonicated and restricted DNA fragments from plasmid clones containing the paramyosin gene, unc-15 in C. elegans were recloned into lac-z expression plasmid vectors. Numerous fragments were recovered which encoded paramyosin epitopes. These clones produced fusion protein which cross-reacted with a polyclonal and three monoclonal antibodies. The samples of sonicated fragments were also used for shot gun sequencing of the gene. Sequenced fragments were aligned with a computer analysis. Amino acid sequence of the expressed clones matched exactly to that of the exon.

Original languageEnglish
Pages (from-to)81-84
Number of pages4
JournalNucleic acids symposium series
Issue number19
Publication statusPublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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