Antigenic and receptor binding properties of enterovirus 68

Tadatsugu Imamura, Okamoto Michiko, Shin Ichi Nakakita, Akira Suzuki, Mariko Saito, Raita Tamaki, Socorro Lupisan, Chandra NathRoy, Hiroaki Hiramatsu, Kan Etsu Sugawara, Katsumi Mizuta, Yoko Matsuzaki, Yasuo Suzuki, Hitoshi Oshitani

Research output: Contribution to journalArticlepeer-review

70 Citations (Scopus)

Abstract

Increased detection of enterovirus 68 (EV68) among patients with acute respiratory infections has been reported from different parts of the world in the late 2000s since its first detection in pediatric patients with lower-respiratory-tract infections in 1962. However, the underlying molecular mechanisms for this trend are still unknown. We therefore aimed to study the antigenicity and receptor binding properties of EV68 detected in recent years in comparison to the prototype strain of EV68, the Fermon strain.We first performed neutralization (NT) and hemagglutination inhibition (HI) tests using antisera generated for EV68 strains detected in recent years.We found that the Fermon strain had lower HI and NT titers than recently detected EV68 strains. The HI and NT titers were also significantly different between strains of different genetic lineages among recently detected EV68 strains. We further studied receptor binding specificities of EV68 strains for sialyloligosaccharides using glycan array analysis. In glycan array analysis, all tested EV68 strains showed affinity for α2-6-linked sialic acids (α2-6 SAs) compared to α2-3 SAs. Our study demonstrates that emergence of strains with different antigenicity is the possible reason for the increased detection of EV68 in recent years. Additionally, we found that EV68 preferably binds to α2-6 SAs, which suggests that EV68 might have affinity for the upper respiratory tract.

Original languageEnglish
Pages (from-to)2374-2384
Number of pages11
JournalJournal of virology
Volume88
Issue number5
DOIs
Publication statusPublished - 2014 Mar

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint Dive into the research topics of 'Antigenic and receptor binding properties of enterovirus 68'. Together they form a unique fingerprint.

Cite this