Antigen-induced airway responses are inhibited by a potassium channel opener

M. Ichinose, M. Miura, T. Takahashi, H. Yamauchi, N. Nakajima, A. Igarashi, J. Ishikawa, H. Inoue, K. Maeyama, T. Watanabe, T. Takishima, K. Shirato

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3 Citations (Scopus)

Abstract

We have investigated the effect of a potassium channel opener, BRL 38227, on antigen-induced bronchoconstriction and airway microvascular leakage in sensitized guinea pigs by simultaneously measuring pulmonary resistance (RL) and extravasation of Evans blue dye. Guinea pigs were sensitized 3 wk before experimentation with ovalbumin (OA) and aluminum hydroxide. The trachea was cannulated, and lungs were mechanically ventilated. All animals were pretreated 30 min before experimentation with atropine (1 mg/kg intravenously) and propranolol 1 mg/kg to block muscarinic and β-adrenergic responses, respectively. BRL 38227 (200 μg/kg) was administered intravenously 1 min before intravenous dye injection (30 mg/kg); OA (3 mg/ml) was inhaled using an ultrasonic nebulizer (for 30 s) 1 min after dye injection. BRL 38227 significantly inhibited OA-induced bronchoconstrictor response (p < 0.01) and plasma leakage in trachea (p < 0.05) and main bronchi (p < 0.05). BRL 38227 also had an inhibitory effect on exogenous histamine- and leukotriene-induced bronchoconstriction and microvascular leakage. However, BRL 38227 did not affect OA-induced histamine release from minced lung tissues in sensitized guinea pigs. We conclude that the allergic bronchoconstrictor response and airway plasma leakage are inhibited by a potassium channel opener, possibly as a result of its effect on the airway smooth muscle and the postcapillary venule level.

Original languageEnglish
Pages (from-to)388-393
Number of pages6
JournalAmerican journal of respiratory and critical care medicine
Volume150
Issue number2
DOIs
Publication statusPublished - 1994

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

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