Antifibrotic effect of edaravone in rat liver cirrhosis induced by dimethylnitrosamine

Hiroto Tanaka, Hiroki Ueda, Hiroko Fukuchi, Masakazu Ichinose

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Edaravone (EDA), a newly synthesized free radical scavenger, has shown excellent results in the treatment of stroke. An overproduction of reactive oxygen species (ROS) causing oxidative DNA damage has been accounted as a major factor causing liver injury and fibrosis. Therefore, we examined its effect of EDA in rat model of liver cirrhosis induced by dimethylnitrosamine (DMN). Ten rats (DMN-group) were injected intraperitoneally with DMN (10 μg/g body weight) alone and another ten rats (EDA-group) were injected intraperitoneally with EDA (10 mg/kg body weight) 2 h after being injected with DMN. Both groups underwent their injection regimen three times a week for 4 weeks, after which the rats were sacrificed and their liver tissue sections were stained with Azan-Mallory for quantitative analyses of fibrosis development, using soft imaging and a previously published scoring system. Additionally, these sections were immunohistochemically stained using an antibody against α-smooth muscle actin (α-SMA). The total-bililubin in the EDA-group was found to be lower than that in the DMN-group. Quantitive analysis of liver fibrosis showed that the fibrotic area of the EDA-group was significantly smaller than that of the DMN-group. Additionally, the number of α-SMA positive cells in the EDA-group were significantly lower than that in the DMN-group. This study showed that EDA reduces liver fibrosis in a rat of cirrhosis induced by DMN. These data suggest that the reduction of liver fibrosis by EDA may be induced by the suppression of activated hepatic stellate cells.

Original languageEnglish
Pages (from-to)229-233
Number of pages5
JournalClinical and Experimental Medicine
Volume9
Issue number3
DOIs
Publication statusPublished - 2009 Sep 1

Keywords

  • Chronic liver disease
  • Dimethylnitrosamine
  • Edaravone
  • Liver cirrhosis
  • Liver fibrosis
  • Radical scavenger
  • Reactive oxygen species (ROS)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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