Antibodies inactivating mGluR1 metabotropic glutamate receptor block long-term depression in cultured Purkinje cells

Ryuichi Shigemoto, Takaaki Abe, Sakashi Nomura, Shigetada Nakanishi, Tomoo Hirano

Research output: Contribution to journalArticle

211 Citations (Scopus)

Abstract

Antibodies were raised against two distinct extracellular sequences of the rat mGIuR1 metabotropic glutamate receptor expressed as bacterial fusion proteins. Both antibodies specifically reacted with mGIuR1 in the rat cerebellum and inhibited the mGIuR1 activity as assessed by the analysis of glutamate-stimulated inositol phosphate formation in CHO cells expressing mGIuR1. Using these antibodies, we examined the role of mGIuR1 in the induction of long-term depression in cultured Purkinje cells. In voltage-clamped Purkinje cells, current induced by iontophoretically applied glutamate was persistently depressed by depolarization of the Purkinje cells in conjunction with the glutamate application. The mGIuR1 antibodies completely blocked the depression of glutamate-induced current. The results indicate that activation of mGIuR1 is necessary for the induction of cerebellar long-term depression and that these mGIuR1 antibodies can be used as selective antagonists.

Original languageEnglish
Pages (from-to)1245-1255
Number of pages11
JournalNeuron
Volume12
Issue number6
DOIs
Publication statusPublished - 1994 Jun
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)

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