Anti-podoplanin Monoclonal Antibody LpMab-7 Detects Metastatic Lesions of Osteosarcoma

Mika K. Kaneko, Hiroharu Oki, Satoshi Ogasawara, Michiaki Takagi, Yukinari Kato

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Osteosarcoma is the most common primary malignant bone tumor and is highly metastatic to the lungs. Therefore, the development of a novel molecular targeting therapy against metastasis of osteosarcoma is necessary. A platelet aggregation-inducing factor, podoplanin/aggrus, is involved in tumor metastasis. Furthermore, podoplanin expression was reported to be involved in the poor prognosis of osteosarcoma patients. However, the association between podoplanin expression and metastasis of osteosarcoma remains unclear because of the lack of highly sensitive anti-podoplanin monoclonal antibodies (MAbs). In this study, we used a novel anti-podoplanin MAb, LpMab-7, which is more sensitive than well-known anti-podoplanin MAbs in immunohistochemistry. Immunohistochemical analysis using LpMab-7 showed that podoplanin expression at primary lesions is observed in 15 out of 16 (93.8%) cases. Furthermore, podoplanin expression at metastatic lesions was higher compared with primary lesions in three out of four (75%) cases with lung metastasis. Because LpMab-7 has high sensitivity against podoplanin, it is expected to be useful for molecular targeting therapy for osteosarcomas.

Original languageEnglish
Pages (from-to)154-161
Number of pages8
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume34
Issue number3
DOIs
Publication statusPublished - 2015 Jun 1

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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