TY - JOUR
T1 - Anti melanogenic effect of Croton roxburghii and Croton sublyratus leaves in α-MSH stimulated B16F10 cells
AU - Chatatikun, Moragot
AU - Yamauchi, Takeshi
AU - Yamasaki, Kenshi
AU - Aiba, Setsuya
AU - Chiabchalard, Anchalee
N1 - Funding Information:
This study was supported financially by Doctoral Degree Chulalongkorn University 100th Year Birthday Anniversary, Overseas Research Experience Scholarship for Graduate Student and the Research fund project 58002, Faculty of Allied Health Sciences, Chulalongkorn University, Thailand. This research was also supported by the National Research University Project, Office of Higher Education Commission (NRU59-007-HR). We appreciated the assistance of the HRH Princess Sirindhorn Herb Garden to give the plants for this project. Finally, we would like to express gratitude to Prof. Dr. Duncan R. Smith (Institute of Molecular Biosciences, Mahidol University) for his critical reading of this manuscript.
Funding Information:
This study was supported financially by Doctoral Degree Chulalongkorn University 100th Year Birthday Anniversary, Overseas Research Experience Scholarship for Graduate Student and the Research fund project 58002 , Faculty of Allied Health Sciences, Chulalongkorn University , Thailand. This research was also supported by the National Research University Project, Office of Higher Education Commission ( NRU59-007-HR ). We appreciated the assistance of the HRH Princess Sirindhorn Herb Garden to give the plants for this project. Finally, we would like to express gratitude to Prof. Dr. Duncan R. Smith (Institute of Molecular Biosciences, Mahidol University) for his critical reading of this manuscript.
Publisher Copyright:
© 2017 Center for Food and Biomolecules, National Taiwan University
PY - 2019/1
Y1 - 2019/1
N2 - Croton roxburghii and Croton sublyratus have been used as skin treatments in traditional medicine. The objective of the present study was to investigate the antimelanogenic effect of ethanol extracts of Croton roxburghii (CRE) and Croton sublyratus (CSE) leaves on cellular melanin content and cellular tyrosinase activity as mediated by the action of microthalmia transcription factor (MITF) and melanogenic enzymes. Croton roxburghii and Croton sublyratus leaves were extracted by petroleum ether, dichloromethane and absolute ethanol, sequentially. The ethanolic crude extracts were examined for antimelanogenic activity by their ability to decrease melanin content and cellular tyrosinase activity in alpha-melanocyte-stimulating hormone-stimulated B16F10 melanoma cells. In addition, the extracts were evaluated to determine a plausible mechanism of melanogenesis suppression through determining the activation of MITF transcription factor and melanogenic proteins (tyrosinase, tyrosinase-related protein 1 or TRP-1 and tyrosinase-related protein 2 or TRP-2) at the transcriptional and translation levels in α-MSH-induced B16F10 cells. Upon treatment with CRE and CSE, the cells showed significant decreases in melanin content and cellular tyrosinase activity. CRE and CSE also suppressed MITF, tyrosinase, TRP-1 and TRP-2 at the transcription and translation levels in α-MSH-stimulated melanin biosynthesis in B16F10 cells. Our finding shows that CRE and CSE inhibit melanin content and cellular tyrosinase activity through suppressing MITF and melanogenic enzymes. CRE and CSE may be useful to combine with skin whitening agents for cosmetic uses.
AB - Croton roxburghii and Croton sublyratus have been used as skin treatments in traditional medicine. The objective of the present study was to investigate the antimelanogenic effect of ethanol extracts of Croton roxburghii (CRE) and Croton sublyratus (CSE) leaves on cellular melanin content and cellular tyrosinase activity as mediated by the action of microthalmia transcription factor (MITF) and melanogenic enzymes. Croton roxburghii and Croton sublyratus leaves were extracted by petroleum ether, dichloromethane and absolute ethanol, sequentially. The ethanolic crude extracts were examined for antimelanogenic activity by their ability to decrease melanin content and cellular tyrosinase activity in alpha-melanocyte-stimulating hormone-stimulated B16F10 melanoma cells. In addition, the extracts were evaluated to determine a plausible mechanism of melanogenesis suppression through determining the activation of MITF transcription factor and melanogenic proteins (tyrosinase, tyrosinase-related protein 1 or TRP-1 and tyrosinase-related protein 2 or TRP-2) at the transcriptional and translation levels in α-MSH-induced B16F10 cells. Upon treatment with CRE and CSE, the cells showed significant decreases in melanin content and cellular tyrosinase activity. CRE and CSE also suppressed MITF, tyrosinase, TRP-1 and TRP-2 at the transcription and translation levels in α-MSH-stimulated melanin biosynthesis in B16F10 cells. Our finding shows that CRE and CSE inhibit melanin content and cellular tyrosinase activity through suppressing MITF and melanogenic enzymes. CRE and CSE may be useful to combine with skin whitening agents for cosmetic uses.
KW - B16F10 mouse melanoma cells
KW - Croton roxburghii
KW - Croton sublyratus
KW - Melanin
KW - Tyrosinase
UR - http://www.scopus.com/inward/record.url?scp=85046171472&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85046171472&partnerID=8YFLogxK
U2 - 10.1016/j.jtcme.2017.12.002
DO - 10.1016/j.jtcme.2017.12.002
M3 - Article
AN - SCOPUS:85046171472
VL - 9
SP - 66
EP - 72
JO - Journal of Traditional and Complementary Medicine
JF - Journal of Traditional and Complementary Medicine
SN - 2225-4110
IS - 1
ER -