Anti-DNA Idiotypes Deposited in Renal Glomeruli of Patients With Lupus Nephritis

Masahiko Suzuki, Akira Hatakeyama, Junichi Kameoka, Eiichi Tamate, Akira Yusa, Kosei Kurosawa, Takao Saito, Takeshi Sasaki, Kaoru Yoshinaga

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Idiotypes (Id) of human anti-DNA antibodies, designated as O-81 Id, were specifically detected on the immune deposits of renal glomeruli in 46% of patients with lupus nephritis. Id-binding to anti-Id antibodies was blocked by free O-81 Id and to some extent by free DNA. DNase or acid buffer treatment failed to reveal new Id determinants on the deposits. O-81 Id and NE-1 Id activity were also detected on the renal eluate-derived IgG, but not IgM from the autopsy cases with lupus nephritis. The incidences of O-81 Id were not associated with histological features in the glomeruli, but the distribution patterns were similar to those of IgG deposits. Our study also showed that 65% to 70% of patients with IgG deposits either in the subendothelium or in the subepithelial area of the glomerular basement membrane (GBM) showed positive tests for O-81 Id. It was also noted that most patients with massive proteinuria had O-81 Id in their glomeruli. It is concluded that O-81 Id deposits are relatively specific for active lupus nephritis and that immunofluorescence studies using anti-Id antibodies may be clinically useful for specifying the renal lesions of systemic lupus erythematosus (SLE).

Original languageEnglish
Pages (from-to)232-239
Number of pages8
JournalAmerican Journal of Kidney Diseases
Volume18
Issue number2
DOIs
Publication statusPublished - 1991

Keywords

  • Idiotype
  • anti-DNA antibodies
  • immune deposits
  • lupus nephritis

ASJC Scopus subject areas

  • Nephrology

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