Anti-cytoplasmic autoantibodies reactive with epithelial cells of the salivary gland in sera from patients with sjogren's syndrome: Their disease- and organ-specificities

Maya Sakamoto, Masaaki Miyazawa, Shiro Mori, Ryuichi Fujisawa

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

To test whether the autoantibodies reactive with epithelial cells of the salivary gland in sera from Sjogren's syndrome (SS) patients are specific for the organ and the disease, tissue reactivities of serum IgG obtained from the patients with SS and oral lichen planus (OLP), another immune-mediated oral mucosal disease, were examined by immunohistochemistry and Western blotting. IgG purified from the sera of SS patients specifically localized not only on the nuclei but also on the cytoplasm of the salivary gland epithelial cells. On the other hand, no convincing staining of the epithelial cells was observed when IgG purified from the sera of OLP patients or those from healthy controls were used for immunohistochemistry. No cytoplasmic staining was observed when sections of kidneys and pancreas were stained with SS patients' IgG. In Western blotting performed by using lysates of a submandibular gland as antigens, all the IgG prepared from the SS patients reacted prominently with several protein bands, including those specific for the disease and the organ. These results suggest that production of autoantibodies reacting with the cytoplasm of salivary gland epithelial cells is a characteristic of SS, and may play a role in the pathogenesis of the sialadenitis.

Original languageEnglish
Pages (from-to)20-25
Number of pages6
JournalJournal of Oral Pathology and Medicine
Volume28
Issue number1
DOIs
Publication statusPublished - 1999

Keywords

  • Autoantibodies
  • Immunohistochemistry
  • Oral lichen planus
  • Salivary gland
  • Sjogren's syndrome

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Oral Surgery
  • Otorhinolaryngology
  • Cancer Research
  • Periodontics

Fingerprint Dive into the research topics of 'Anti-cytoplasmic autoantibodies reactive with epithelial cells of the salivary gland in sera from patients with sjogren's syndrome: Their disease- and organ-specificities'. Together they form a unique fingerprint.

Cite this