Anti-Bovine Podoplanin Monoclonal Antibody PMab-44 Detects Goat Podoplanin in Immunohistochemistry

Shinji Yamada, Mika K. Kaneko, Yoshikazu Furusawa, Shunsuke Itai, Masato Sano, Takuro Nakamura, Miyuki Yanaka, Saori Handa, Kayo Hisamatsu, Yoshimi Nakamura, Masanori Koyanagi, Masato Fukui, Hiroyuki Harada, Yukinari Kato

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5 Citations (Scopus)

Abstract

Podoplanin (PDPN) is expressed in type I alveolar cells, kidney podocytes, and lymphatic endothelial cells. We have characterized the PDPNs of various animal species using specific anti-PDPN monoclonal antibodies (mAbs). In this study, we investigated whether these anti-PDPN mAbs cross-react with goat PDPN (gPDPN). Flow cytometry demonstrated that the anti-bovine PDPN mAb PMab-44 (IgG1, kappa) reacts with gPDPN, which is overexpressed in CHO-K1 cells. Using immunohistochemical analysis, type I alveolar cells of goat lung were strongly detected by PMab-44. These results indicate that PMab-44 is useful for investigating gPDPN.

Original languageEnglish
Pages (from-to)96-99
Number of pages4
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume38
Issue number2
DOIs
Publication statusPublished - 2019 Apr 1

Keywords

  • PDPN
  • PMab-44
  • goat podoplanin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Yamada, S., Kaneko, M. K., Furusawa, Y., Itai, S., Sano, M., Nakamura, T., Yanaka, M., Handa, S., Hisamatsu, K., Nakamura, Y., Koyanagi, M., Fukui, M., Harada, H., & Kato, Y. (2019). Anti-Bovine Podoplanin Monoclonal Antibody PMab-44 Detects Goat Podoplanin in Immunohistochemistry. Monoclonal antibodies in immunodiagnosis and immunotherapy, 38(2), 96-99. https://doi.org/10.1089/mab.2018.0031