Anti-Allergic Drugs Tranilast and Ketotifen Dose-Dependently Exert Mast Cell-Stabilizing Properties

Asuka Baba, Masahiro Tachi, Yutaka Ejima, Yasuhiro Endo, Hiroaki Toyama, Mitsunobu Matsubara, Kazutomo Saito, Masanori Yamauchi, Chieko Miura, Itsuro Kazama

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Background: Anti-allergic drugs, such as tranilast and ketotifen, inhibit the release of chemokines from mast cells. However, we know little about their direct effects on the exocytotic process of mast cells. Since exocytosis in mast cells can be monitored electrophysiologically by changes in the whole-cell membrane capacitance (Cm), the absence of such changes by these drugs indicates their mast cell-stabilizing properties. Methods: Employing the standard patch-clamp whole-cell recording technique in rat peritoneal mast cells, we examined the effects of tranilast and ketotifen on the Cm during exocytosis. Using confocal imaging of a water-soluble fluorescent dye, lucifer yellow, we also examined their effects on the deformation of the plasma membrane. Results: Relatively lower concentrations of tranilast (100, 250 μM) and ketotifen (1, 10 μM) did not significantly affect the GTP-γ-S-induced increase in the Cm. However, higher concentrations of tranilast (500 μM, 1 mM) and ketotifen (50, 100 μM) almost totally suppressed the increase in the Cm, and washed out the trapping of the dye on the surface of the mast cells. Compared to tranilast, ketotifen required much lower doses to similarly inhibit the degranulation of mast cells or the increase in the Cm. Conclusions: This study provides electrophysiological evidence for the first time that tranilast and ketotifen dose-dependently inhibit the process of exocytosis, and that ketotifen is more potent than tranilast in stabilizing mast cells. The mast cell-stabilizing properties of these drugs may be attributed to their ability to counteract the plasma membrane deformation in degranulating mast cells.

Original languageEnglish
Pages (from-to)15-27
Number of pages13
JournalCellular Physiology and Biochemistry
Volume38
Issue number1
DOIs
Publication statusPublished - 2016 Jan 1

Keywords

  • Exocytosis
  • Ketotifen
  • Mast cell-stabilizing properties
  • Membrane capacitance
  • Plasma membrane deformation
  • Rat peritoneal mast cells
  • Tranilast

ASJC Scopus subject areas

  • Physiology

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