TY - JOUR
T1 - Anterograde labeling of the corticospinal tract in jimpy mutant mice by DiI injection into the motor cortex
AU - Shibata-Iwasaki, Riichi
AU - Dekimoto, Hideyuki
AU - Katsuyama, Yu
AU - Kikkawa, Satoshi
AU - Terashima, Toshio
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2007
Y1 - 2007
N2 - Carbocyanine fluorescent dye, DiI, is an excellent anterograde/retrograde neural tracer, but its efficacy for the anterograde labeling of neural circuits in the adult brain tends to decrease with ages. The present study shows that an injection of DiI into the motor cortex of the young adult jimpy mutant mice (Plp1jp/+) resulted in successful anterograde labeling of corticospinal tract fibers. Furthermore, an injection of Fast Blue into the lumbar spinal cord of the mutant mice resulted in retrograde labeling of layer 5 corticospinal tract neurons within the motor cortex. Since no abnormality except for myelin deficiency is known in the long descending and ascending tracts of jimpy mutant mouse, this mutant is suitable for neural tracing studies of long axonal trajectories with the use of carbocyanine dye, DiI, although these males die between 20 and 40 days of age.
AB - Carbocyanine fluorescent dye, DiI, is an excellent anterograde/retrograde neural tracer, but its efficacy for the anterograde labeling of neural circuits in the adult brain tends to decrease with ages. The present study shows that an injection of DiI into the motor cortex of the young adult jimpy mutant mice (Plp1jp/+) resulted in successful anterograde labeling of corticospinal tract fibers. Furthermore, an injection of Fast Blue into the lumbar spinal cord of the mutant mice resulted in retrograde labeling of layer 5 corticospinal tract neurons within the motor cortex. Since no abnormality except for myelin deficiency is known in the long descending and ascending tracts of jimpy mutant mouse, this mutant is suitable for neural tracing studies of long axonal trajectories with the use of carbocyanine dye, DiI, although these males die between 20 and 40 days of age.
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U2 - 10.1679/aohc.70.297
DO - 10.1679/aohc.70.297
M3 - Article
C2 - 18431030
AN - SCOPUS:42649097255
VL - 70
SP - 297
EP - 301
JO - Archives of Histology and Cytology
JF - Archives of Histology and Cytology
SN - 0914-9465
IS - 5
ER -