Anisotropic distribution of emitting transition dipoles in chlorosome from Chlorobium tepidum: Fluorescence polarization anisotropy study of single chlorosomes

Yutaka Shibata, Yoshitaka Saga, Hitoshi Tamiaki, Shigeru Itoh

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The polarization anisotropy of fluorescence spectra from single chlorosomes isolated from a green sulfur bacterium, Chlorobium (Cb.) tepidum, was observed at 13 K. As the polarizer was rotated, the intensities of the fluorescence bands of both bacteriochlorophyll (BChl)-c self-aggregates and BChl-a in baseplate proteins showed clear oscillations. From the oscillation, the values of the degree of polarization (DP) and the phase shift (PS) between the BChl-c and BChl-a bands were determined for each single chlorosome. The DP versus PS plot for Cb. tepidum chlorosomes showed linear correlations between the PS and the DP values for both BChl-c and BChl-a fluorescence bands. This tendency could be explained from a simulation assuming a random orientation of chlorosomes and a triaxial orientation distribution of emitting transition dipoles within a single chlorosome. The intensity ratios among the X-/Y-/Z-principal transition dipoles were estimated to be 0.3/0.5/1 and 1/0.6/0.1 for the BChl-c and BChl-a fluorescence bands, respectively. Here, the X-, Y-, and Z-axes are perpendicular, parallel to the cytoplasmic membrane, and parallel to the chlorosome long axis, respectively. A theoretical calculation based on the exciton theory was conducted to reproduce the observed triaxial orientation distribution of emitting transition dipoles. The simulation revealed that a deformation introduced to the circular cross section of the rod-shaped BChl-c self-aggregates could qualitatively reproduce results of this study.

Original languageEnglish
Pages (from-to)67-78
Number of pages12
JournalPhotosynthesis Research
Volume100
Issue number2
DOIs
Publication statusPublished - 2009 May 1
Externally publishedYes

Keywords

  • Bacteriochlorophyll-c
  • Confocal microscope
  • Fluorescence polarization anisotropy
  • Green photosynthetic bacteria
  • Photosynthetic antenna complex
  • Single molecule spectroscopy

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

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