A new fluorescent sensor consisting of Cd"-cylcen appended aminocoumarin and a substrate peptide for protein kinase A (PKA) has been designed. Upon phosphorylation by PKA, the metal complex moiety binds to a phosphorylated residue, which In turn displaces the coumarin fluorophore, and this event results In ratiometric change of excitation spectrum In neutral aqueous solution.
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Organic Chemistry