Anion sensor-based ratiometric peptide probe for protein kinase activity

Kazuya Kikuchi, Shigeki Hashimoto, Shin Mizukami, Tetsuo Nagano

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

A new fluorescent sensor consisting of Cd"-cylcen appended aminocoumarin and a substrate peptide for protein kinase A (PKA) has been designed. Upon phosphorylation by PKA, the metal complex moiety binds to a phosphorylated residue, which In turn displaces the coumarin fluorophore, and this event results In ratiometric change of excitation spectrum In neutral aqueous solution.

Original languageEnglish
Pages (from-to)2732-2735
Number of pages4
JournalOrganic letters
Volume11
Issue number13
DOIs
Publication statusPublished - 2009 Jul 2
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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