Angiotensin II can regulate gene expression by the AP-1 binding sequence via a protein kinase C-dependent pathway

Kazuhisa Takeuchi, Norifumi Nakamura, Nigel S. Cook, Richard E. Pratt, Victor J. Dzau

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

An expression vector containing three copies of the AP-1 binding element (TRE) upsteam of a thymidine kinase promotor which controlled the expression of the chloramphenicol acetyl transferase (CAT) gene was transiently transfected into vascular smooth muscle (VSM) cells and a human hepatocarcinoma cell line, Hep G2. Twelve hours of angiotensin (Ang) II exposure stimulated significantly CAT expression by 3.4 fold and 2.7 fold in Hep G2 and VSM cells, respectively. AngII had no effect on CAT expression of a control vector. This AngII-induced stimulation was attenuated significantly by an AngII receptor antagonist, Sar1Ile8 AngII, and abolished completely by a PKC inhibitor, staurosporine. Our data suggest that the TRE plays a crucial role in AngII-induced gene expression that is mediated by PKC. We concluded that TRE is one of the AngII-responsive elements.

Original languageEnglish
Pages (from-to)1189-1194
Number of pages6
JournalBiochemical and biophysical research communications
Volume172
Issue number3
DOIs
Publication statusPublished - 1990 Nov 15

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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