Angiogenesis inhibition by transdominant mutant Ets-1

Toru Nakano, Mayumi Abe, Katsuhiro Tanaka, Ryuzaburo Shineha, Susumu Satomi, Yasufumi Sato

Research output: Contribution to journalArticlepeer-review

58 Citations (Scopus)

Abstract

The expression of transcription factor Ets-1 is induced in endothelial cells (ECs) by angiogenic factor; and in turn Ets-1 converts ECs to angiogenic invasive phenotype. In order to control angiogenesis, we constructed a transdominant mutant Ets-1 (TMEts-1) which acts as a dominant negative molecule. This molecule inhibited the DNA binding and the transactivation activity of the wild-type Ets-1. Stable transfection of murine endothelial cell line MSS31 cells with the TMets-1 gene impaired angiogenic activities including proliferation, migration, invasion, and tube formation in type-1 collagen gel. Finally, we incorporated the TMets-1 gene into a non-proliferative adenovirus vector, designated as AdTMets-1. AdTMets- 1 significantly inhibited angiogenesis in the Matrigel plugs injected into the subcutaneous tissue of C57BL mice. These results indicate that TMets-1 would be a tool for angiogenic inhibition. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)255-262
Number of pages8
JournalJournal of Cellular Physiology
Volume184
Issue number2
DOIs
Publication statusPublished - 2000

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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