Anchoring protein crystals to mounting loops with hydrogel using inkjet technology

Akira Shinoda, Yoshikazu Tanaka, Min Yao, Isao Tanaka

Research output: Contribution to journalArticlepeer-review

Abstract

X-ray crystallography is an important technique for structure-based drug discovery, mainly because it is the only technique that can reveal whether a ligand binds to the target protein as well as where and how it binds. However, ligand screening by X-ray crystallography involves a crystal-soaking experiment, which is usually performed manually. Thus, the throughput is not satisfactory for screening large numbers of candidate ligands. In this study, a technique to anchor protein crystals to mounting loops by using gel and inkjet technology has been developed; the method allows soaking of the mounted crystals in ligand-containing solution. This new technique may assist in the design of a fully automated drug-screening pipeline.

Original languageEnglish
Pages (from-to)2794-2799
Number of pages6
JournalActa Crystallographica Section D: Biological Crystallography
Volume70
Issue number11
DOIs
Publication statusPublished - 2014 Nov 1

Keywords

  • hydrogel
  • inkjet
  • mounting

ASJC Scopus subject areas

  • Structural Biology

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