TY - JOUR
T1 - Analysis on the promoter region of human decidual prolactin gene in the progesterone-induced decidualization and cAMP-induced decidualization of human endometrial stromal cells
AU - Wang, Dong Fang
AU - Minoura, Hiroyuki
AU - Sugiyama, Takashi
AU - Tanaka, Keisuke
AU - Kawato, Hiroaki
AU - Toyoda, Nagayasu
AU - Sagawa, Norimasa
PY - 2007/6
Y1 - 2007/6
N2 - Purpose: To elucidate the promoter region of human decidual prolactin (dPRL) gene in the human endometrial stromal cells (ESC). Methods: Various segments of the human dPRL promoter that direct the expression of the secreted alkaline phosphatase (SEAP) reporter gene were transfected into human ESC decidualized by estrogen (E) + progesterone (P) or cyclic AMP (cAMP) to identify E + P or cAMP responsive elements. Results: The region between nucleotides 2038 and 1605 relative to the transcriptional initiation site includes two activator protein-1 (AP-1) sites, which both provided maximal response to E + P or cAMP in decidualized cells. When either AP-1 site was mutated, response in the promoter activity to both E + P or cAMP response showed a decrease compared with control. The region between 310 and 285 that contains consensus-binding sequences for transcription factors of CCAAT/ Enhancer-binding proteins (C/EBP) contributed to E + P and cAMP response in decidualized cells. Also, the 5′-flanking region that extends 79 base pairs upstream, including an imperfect cAMP response element (CRE), contributed to E + P and cAMP response. In cells treated with E + P or cAMP for 10 days, mutant of C/EBP-binding site showed an increase in promoter activity comparing to dPRL-2038. In contrast, treatment with PKI showed a decrease in promoter activity in cells treated with E + P or cAMP alone. Conclusions: These results suggest that cAMP-induced region of the human dPRL promoter resides between -1862 and -1856, -1703 and -1697, -310 and -285, and that the sequences between -1862 and -1856, -1703 and -1697 of the promoter display E + P-induced promoter activity. Furthermore, the current study indicates that E + P or cAMP cooperatively regulate the dPRL gene transcription through some transcriptional factors such as C/EBP, CREB, and other cofactor(s), and that some repressor(s) or corepressor(s) may be involved in the C/EBP-binding site of the human dPRL promoter.
AB - Purpose: To elucidate the promoter region of human decidual prolactin (dPRL) gene in the human endometrial stromal cells (ESC). Methods: Various segments of the human dPRL promoter that direct the expression of the secreted alkaline phosphatase (SEAP) reporter gene were transfected into human ESC decidualized by estrogen (E) + progesterone (P) or cyclic AMP (cAMP) to identify E + P or cAMP responsive elements. Results: The region between nucleotides 2038 and 1605 relative to the transcriptional initiation site includes two activator protein-1 (AP-1) sites, which both provided maximal response to E + P or cAMP in decidualized cells. When either AP-1 site was mutated, response in the promoter activity to both E + P or cAMP response showed a decrease compared with control. The region between 310 and 285 that contains consensus-binding sequences for transcription factors of CCAAT/ Enhancer-binding proteins (C/EBP) contributed to E + P and cAMP response in decidualized cells. Also, the 5′-flanking region that extends 79 base pairs upstream, including an imperfect cAMP response element (CRE), contributed to E + P and cAMP response. In cells treated with E + P or cAMP for 10 days, mutant of C/EBP-binding site showed an increase in promoter activity comparing to dPRL-2038. In contrast, treatment with PKI showed a decrease in promoter activity in cells treated with E + P or cAMP alone. Conclusions: These results suggest that cAMP-induced region of the human dPRL promoter resides between -1862 and -1856, -1703 and -1697, -310 and -285, and that the sequences between -1862 and -1856, -1703 and -1697 of the promoter display E + P-induced promoter activity. Furthermore, the current study indicates that E + P or cAMP cooperatively regulate the dPRL gene transcription through some transcriptional factors such as C/EBP, CREB, and other cofactor(s), and that some repressor(s) or corepressor(s) may be involved in the C/EBP-binding site of the human dPRL promoter.
KW - Decidual prolactin
KW - Decidualization
KW - Progesterone
KW - Transcriptional regulation
KW - cAMP
UR - http://www.scopus.com/inward/record.url?scp=34250003258&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34250003258&partnerID=8YFLogxK
U2 - 10.1007/s11010-006-9388-z
DO - 10.1007/s11010-006-9388-z
M3 - Article
C2 - 17187171
AN - SCOPUS:34250003258
VL - 300
SP - 239
EP - 247
JO - Enzymologia
JF - Enzymologia
SN - 0300-8177
IS - 1-2
ER -