Analysis of the subcellular localization of the human histone methyltransferase SETDB1

Keisuke Tachibana, Eiko Gotoh, Natsuko Kawamata, Kenji Ishimoto, Yoshie Uchihara, Hiroko Iwanari, Akira Sugiyama, Takeshi Kawamura, Yasuhiro Mochizuki, Toshiya Tanaka, Juro Sakai, Takao Hamakubo, Tatsuhiko Kodama, Takefumi Doi

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

SET domain, bifurcated 1 (SETDB1) is a histone methyltransferase that methylates lysine 9 on histone H3. Although it is important to know the localization of proteins to elucidate their physiological function, little is known of the subcellular localization of human SETDB1. In the present study, to investigate the subcellular localization of hSETDB1, we established a human cell line constitutively expressing enhanced green fluorescent protein fused to hSETDB1. We then generated a monoclonal antibody against the hSETDB1 protein. Expression of both exogenous and endogenous hSETDB1 was observed mainly in the cytoplasm of various human cell lines. Combined treatment with the nuclear export inhibitor leptomycin B and the proteasome inhibitor MG132 led to the accumulation of hSETDB1 in the nucleus. These findings suggest that hSETDB1, localized in the nucleus, might undergo degradation by the proteasome and be exported to the cytosol, resulting in its detection mainly in the cytosol.

Original languageEnglish
Pages (from-to)725-731
Number of pages7
JournalBiochemical and biophysical research communications
Volume465
Issue number4
DOIs
Publication statusPublished - 2015 Oct 2
Externally publishedYes

Keywords

  • Histone methyltransferase
  • Monoclonal antibody
  • Protein localization
  • SETDB1

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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