Analysis of the regulation of actin cytoskeleton dynamics using dronpa, a photochromic fluorescent protein

Kazumasa Ohashi, Tai Kiuchi, Tomoaki Nagai, Kensaku Mizuno

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cofllin stimulates actin filament disassembly by severing and depolymerizing actin filaments and accelerates actin filament turnover. It is unclear whether cofilin contributes to stimulus-induced actin filament assembly by supplying actin monomers for polymerization or by creating free barbed ends through its severing activity. By measuring the time-lapse fluorescence decay of photoactivated Dronpa-actin, we have assessed the cytoplasmic actin monomer pool in living cells and provide evidence that cofllin is involved in production of more than half of the actin monomers in the cytoplasm. Actin monomers in the cytoplasm were incorporated into the tip of the lamellipodium, and incorporation depended both on cofllin activity and on the size of the cytoplasmic actin monomer pool. We therefore propose that cofllin critically contributes to stimulus-induced actin filament assembly and lamellipodium formation by supplying actin monomers abundantly to the cytoplasm.

Original languageEnglish
Title of host publication2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"
Pages317-322
Number of pages6
DOIs
Publication statusPublished - 2008 Dec 1
Event2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation" - Nagoya, Japan
Duration: 2008 Nov 62008 Nov 9

Publication series

Name2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008

Other

Other2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation"
CountryJapan
CityNagoya
Period08/11/608/11/9

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition
  • Human-Computer Interaction
  • Electrical and Electronic Engineering

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