Analysis of the Promoter Activity of the Taka-Amylase Gene and the Phosphoglycerate Kinase Gene in a Shoyu-koji Mold Aspergillus oryzae KBN616

Tetsuya Kimura, Takashi Makino, Takeshi Aburatani, Shuichi Karita, Kazuo Sakka, Kunio Ohmiya

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Promoter activity of the genes encoding Taka-amylase A and phosphoglycerate kinase of Aspergillus oryzae was analyzed using Escherichia coli β-glucuronidase as a reporter in a shoyu-koji mold A. oryzae KBN616. Assay of the β-glucuronidase extracted from the mycelia of the transformants grown in the media containing different carbon sources suggests that expression of the Taka-amylase A gene was not induced by starch in A. oryzae KBN616. Analysis of proteins in the culture supernatant of A. oryzae KBN616 after cultivation in starch medium supports this result. The phosphoglycerate kinase gene of A. oryzae KBN616 was shown to be expressed constitutively in the medium containing glucose and starch.

Original languageEnglish
Pages (from-to)44-47
Number of pages4
JournalFood Science and Technology Research
Volume6
Issue number1
DOIs
Publication statusPublished - 2000 Feb
Externally publishedYes

Keywords

  • Aspergillus oryzae
  • Phosphoglycerate kinase
  • Promoter
  • Shoyu-koji
  • Taka-amylase

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering
  • Marketing

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