Analysis of the molecular evolution of histone variant H2A.Z using a linker-mediated complex strategy and yeast genetic complementation

Saho Kitagawa; Masayuki Kusakabe; Daisuke Takahashi; Takumi Narimiya; Yu Nakabayashi; Masayuki Seki; Chihiro Horigome; Masahiko Harata

doi:10.1093/bbb/zbab190

Analysis of the molecular evolution of histone variant H2A.Z using a linker-mediated complex strategy and yeast genetic complementation

Saho Kitagawa, Masayuki Kusakabe, Daisuke Takahashi, Takumi Narimiya, Yu Nakabayashi, Masayuki Seki, Chihiro Horigome, Masahiko Harata

Research output: Contribution to journal › Article › peer-review

Abstract

The histone variant H2A.Z is deposited into chromatin by specific machinery and is required for genome functions. Using a linker-mediated complex strategy combined with yeast genetic complementation, we demonstrate evolutionary conservation of H2A.Z together with its chromatin incorporation and functions. This approach is applicable to the evolutionary analyses of proteins that form complexes with interactors.

Original language	English
Pages (from-to)	104-108
Number of pages	5
Journal	Bioscience, Biotechnology and Biochemistry
Volume	86
Issue number	1
DOIs	https://doi.org/10.1093/bbb/zbab190
Publication status	Published - 2022 Jan 1

Keywords

evolutionary conservation
histone variant H2A.Z
yeast genetic complementation

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1093/bbb/zbab190

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AU - Kitagawa, Saho

AU - Kusakabe, Masayuki

AU - Takahashi, Daisuke

AU - Narimiya, Takumi

AU - Nakabayashi, Yu

AU - Seki, Masayuki

AU - Horigome, Chihiro

AU - Harata, Masahiko

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AB - The histone variant H2A.Z is deposited into chromatin by specific machinery and is required for genome functions. Using a linker-mediated complex strategy combined with yeast genetic complementation, we demonstrate evolutionary conservation of H2A.Z together with its chromatin incorporation and functions. This approach is applicable to the evolutionary analyses of proteins that form complexes with interactors.

KW - evolutionary conservation

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Analysis of the molecular evolution of histone variant H2A.Z using a linker-mediated complex strategy and yeast genetic complementation

Abstract

Keywords

ASJC Scopus subject areas

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