Analysis of the modification site in a small molecule-modified peptide by ion trap/time-of-flight hybrid mass spectrometry

Nariyasu Mano, Mio Kamota, Yusuke Inohana, Shin Ichi Yamaguchi, Junichi Goto

Research output: Contribution to journalReview articlepeer-review

7 Citations (Scopus)

Abstract

Ion trap/time-of-flight hybrid mass spectrometers are powerful tools for the detailed structural analysis of modified peptides. We have analyzed Met-Lys-bradykinin modified with deoxycholate at the amino-terminus or the ε-amino group as model peptides. These two modified peptides produced fragment ions with the same nominal but different exact masses in tandem mass spectrometry with low-energy collision-induced dissociation. Accurate high-resolution analysis coupled with MS3 allowed us to distinguish the deoxycholate modification sites in the modified peptides.

Original languageEnglish
Pages (from-to)682-688
Number of pages7
JournalAnalytical and Bioanalytical Chemistry
Volume386
Issue number3
DOIs
Publication statusPublished - 2006 Oct 1

Keywords

  • Ion trap
  • MS analysis
  • Mass spectometry
  • Modification site
  • Small molecule-modified peptide
  • Time-of-flight

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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