Abstract
We established a novel allergic dermatitis model in mouse ear lobes in which antigen-nonspecific inflammation was induced by painting 12-O-tetradecanoylphorbol 13-acetate (TPA) between sensitization and challenge with picryl chloride (PiCl). This model has an advantage for analyzing atopic dermatitis-like inflammation within a short period. Analysis of the time course changes in the PiCl-induced swelling showed that the allergic inflammation was shifted from a delayed-type response to a biphasic response consisting of a weak immediatephase response and a late-phase response by painting with TPA. The application of TPA increased the PiCl-induced infiltration of eosinophils and mast cells at the inflammatory site and shifted the cytokine milieu from Th1 to Th2. The expression of the Th2-inducing cytokine thymic stromal lymphopoietin (TSLP) mRNA was also increased by TPA. These findings suggested that the induction of antigen-nonspecific inflammation by TPA before the antigen challenge enhanced the Th2 response and modified the PiCl-induced delayed type-hypersensitivity. Using this model, we clarified that histamine plays significant roles in the early-phase swelling via H1 receptors and the late-phase swelling via H3/H4 receptors. Thus, we suggested the usefulness of the combined treatment with an H1 antagonist and an H4 antagonist for the suppression of atopic dermatitis.
Original language | English |
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Pages (from-to) | 245-250 |
Number of pages | 6 |
Journal | Journal of Pharmacological Sciences |
Volume | 110 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2009 |
Keywords
- 12-O-tetradecanoylphorbol 13-acetate
- Allergic dermatitis
- Allergic inflammation
- Histamine
- Picryl chloride
- Thymic stromal lymphopoietin
ASJC Scopus subject areas
- Molecular Medicine
- Pharmacology