Analysis of the leukotriene D4 receptor in the granulation tissue of allergic inflammation in rats

Noriyasu Hirasawa, Tυmonori Hoshi, Michiko Kudoh, Suetsugu Mue, Susumu Tsurufuji, Masako Watanabe, Kazuo Ohuchi

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Leukotriene (LT) D4 receptor in the granulation tissue formed in the air pouch-type allergic inflammation model in rats was analyzed. Membrane preparation of the granulation tissue obtained 3-9 days after the antigen challenge has specific binding sites of [3H]LTD4. Scatchard analysis showed that the affinity (Kd) and the density (Bmax) were not changed among the granulation tissue obtained 3-9 days after the antigen challenge. The Kd value in the granulation tissue (0.90 ± 0.12 nM) was close to that in the rat lung (1.00 ± 0.24 nM) and the guinea pig lung (0.86 nM). On the other hand, Bmax (62 ±8 fmol/mg protein) in the granulation tissue was higher than that in the rat lung (21 ±4 fmol/mg protein) but was far less than that in the guinea pig lung (405 fmol/mg protein). LTC4 and LTE4 inhibited the binding of [3H]LTD4 to the membrane preparation of the granulation tissue in a concentration-dependent manner. IC50 of LTQ and LTE4were 1 × 10-7and 2 × 10-7 M, respectively. A guanine nucleotide, guanyl-5′-yl-imido-diphosphate (GppNHp), reduced [3H]LTD4 binding to the membrane preparation of the granulation tissue suggesting that LTD4 receptors in the granulation tissue are associated with G proteins. These results indicate that LTD4 binding sites in the granulation tissue arc high affinity receptors for LTD4. A possible role of LTD4 in the recurrence of allergic inflammation in the chronic phase is discussed.

Original languageEnglish
Pages (from-to)107-111
Number of pages5
JournalInternational archives of allergy and immunology
Volume99
Issue number1
DOIs
Publication statusPublished - 1992 Jan 1

Keywords

  • Allergic inflammation
  • Binding assay
  • G-protein
  • Granulation tissue
  • Leukotriene C
  • Leukotriene D
  • Leukotriene D receptor
  • Leukotriene E
  • Rat lung membrane fraction

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Analysis of the leukotriene D<sub>4</sub> receptor in the granulation tissue of allergic inflammation in rats'. Together they form a unique fingerprint.

Cite this