Analysis of the interaction between human TLR3 ectodomain and nucleic acids.

Kotaro Fukuda, Tadayuki Tsujita, Misako Matsumoto, Tsukasa Seya, Hiroshi Sakiyama, Fumiko Nishikawa, Satoshi Nishikawa, Tsunemi Hasegawa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Toll-like receptor 3 (TLR3) recognizes dsRNA of viral origin and polyriboinosine-polyribocytidylic acid (poly (I:C)). TLR3 mediates the activation of IRF-3 and NF-kappaB and thereby the secretion of type I interferons and inflammatory cytokines. However, the mechanism of this activation is poorly understood. To study the molecular recognition events and biochemical interactions between TLR3 and dsRNA, human TLR3 ectodomain (ECD) fused with a signal peptide at the N-terminus and 6 x His-tag at the C-terminus (TLR3-ECD) was obtained by the baculovirus expression system. To examine the various nucleic acids binding to TLR3-ECD in vitro, a filter binding assay was carried out at pH 4.2-7.6. Interaction of TLR3-ECD with various nucleic acids (particularly dsRNA, in vitro transcripts of tRNA and HCV NS3 aptamer) required an acidic pH.

Original languageEnglish
Pages (from-to)249-250
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number50
DOIs
Publication statusPublished - 2006

ASJC Scopus subject areas

  • Medicine(all)

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