Analysis of the Hemolymph Proteins from the Acorn Barnacle Megabalanus rosa by Two-dimensional Gel Electrophoresis

Koji Muramoto, Tomoko Kuriki, Kiyoshi Nokihara, Hiroshi Yako, Hisao Kamiya

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    The hemolymph proteins of the acorn barnacle Megabalanus rosa were separated by two-dimensional gel electrophoresis. More than 50 proteins were observed in the region of acidic (pl: 4-7) with masses of 18-120 kDa. The proteins separated on the gel were electroblotted onto a polyvinylidene difluoride membrane and were sequenced. Several major proteins were further characterized by amino acid analysis and peptide mapping, and identified to be multiple lectins.

    Original languageEnglish
    Pages (from-to)435-438
    Number of pages4
    JournalFisheries Science
    Volume62
    Issue number3
    DOIs
    Publication statusPublished - 1996 Jun

    Keywords

    • Amino acid sequence
    • Barnacle
    • Hemolymph protein
    • Lectins
    • Two-dimensional gel electrophoresis

    ASJC Scopus subject areas

    • Aquatic Science

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