Analysis of the full-length genome of hepatitis B virus in the serum and cerebrospinal fluid of a patient with acute hepatitis B and transverse myelitis

Jun Inoue, Yoshiyuki Ueno, Takayuki Kogure, Futoshi Nagasaki, Osamu Kimura, Noriyuki Obara, Osamu Kido, Yu Nakagome, Eiji Kakazu, Yasunori Matsuda, Koji Fukushima, Haruna Segawa, Ichiro Nakajima, Yasuto Itoyama, Masaharu Takahashi, Hiroaki Okamoto, Tooru Shimosegawa

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14 Citations (Scopus)

Abstract

Although many extrahepatic manifestations have been described in patients with acute or chronic hepatitis B, there are few reports about neurological disorders. We describe a 55-year-old man who contracted acute hepatitis B virus (HBV) infection and transverse myelitis. His neurological findings were gradually reduced along with the recovery from hepatitis. The cerebrospinal fluid (CSF) was revealed to be positive for HBsAg and HBV DNA. Full-length sequences of HBV in his serum and CSF were determined, and it was revealed that these two isolates had mutations at nucleotide (nt) 1762/1764 in the core promoter region and nt 1896 in the precore region. They were identical to each other except for two ambiguous codes at nt 2020 and 2631 in the CSF isolate. After cloning of the amplicons, substitutions at nt 2020 and 2631 were found in 6 (38%) of the 16 CSF clones. One clone of the 6 CSF clones had an additional substitution at nt 2119. These substitutions were not found in 16 serum clones. The presence of HBV clones unique to CSF suggests that HBV was a possible causative agent of the myelitis.

Original languageEnglish
Pages (from-to)301-304
Number of pages4
JournalJournal of Clinical Virology
Volume41
Issue number4
DOIs
Publication statusPublished - 2008 Apr

Keywords

  • Acute hepatitis B
  • Cerebrospinal fluid
  • Full-length genome
  • Hepatitis B virus
  • Sequence analysis
  • Transverse myelitis

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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    Inoue, J., Ueno, Y., Kogure, T., Nagasaki, F., Kimura, O., Obara, N., Kido, O., Nakagome, Y., Kakazu, E., Matsuda, Y., Fukushima, K., Segawa, H., Nakajima, I., Itoyama, Y., Takahashi, M., Okamoto, H., & Shimosegawa, T. (2008). Analysis of the full-length genome of hepatitis B virus in the serum and cerebrospinal fluid of a patient with acute hepatitis B and transverse myelitis. Journal of Clinical Virology, 41(4), 301-304. https://doi.org/10.1016/j.jcv.2008.01.002