Abstract
To clarify the mechanism of anesthesics, we utilized mutants of Drosophila melanogaster which had a P-element. 1. As for the mutant para838-2-14 of a P-element insertion in the 1st chromosome, we inferred that the cause of hypersensitivity to diethylether was the P-element insertion in the second intron of the para locus (a Na+ channel gene) and not the two base substitutions of the third exon of the gene. 2. We selected one ether-sensitive strain (s3754) and three ether-resistant strains (R3914, R3935, R3944) from the mutants of a P-element insertion in the 2nd or 3rd chromosome, cloned the genomic DNA fragments flanking the P-element insertion sites by plasmid rescue method and sequenced them. The 46.6%, 52.4% and 50.0% homology to the Na+ channel gene of para locus were found in s3754, R3914 and R3944, respectively.
Original language | English |
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Pages (from-to) | 265-268 |
Number of pages | 4 |
Journal | Anesthesia and Resuscitation |
Volume | 30 |
Issue number | 3 |
Publication status | Published - 1994 |
Externally published | Yes |
Keywords
- Drosophila
- P-element
- anesthesia
- mutant
- sodium channel
ASJC Scopus subject areas
- Emergency Medicine
- Anesthesiology and Pain Medicine