Analysis of the complete plastid genome of the unicellular red alga Porphyridium purpureum

Naoyuki Tajima, Shusei Sato, Fumito Maruyama, Ken Kurokawa, Hiroyuki Ohta, Satoshi Tabata, Kohsuke Sekine, Takashi Moriyama, Naoki Sato

    Research output: Contribution to journalArticle

    33 Citations (Scopus)

    Abstract

    We determined the complete nucleotide sequence of the plastid genome of the unicellular marine red alga Porphyridium purpureum strain NIES 2140, belonging to the unsequenced class Porphyridiophyceae. The genome is a circular DNA composed of 217,694 bp with the GC content of 30.3 %. Twenty-nine of the 224 protein-coding genes contain one or multiple intron(s). A group I intron was found in the rpl28 gene, whereas the other introns were group II introns. The P. purpureum plastid genome has one non-coding RNA (ncRNA) gene, 29 tRNA genes and two nonidentical ribosomal RNA operons. One rRNA operon has a tRNAAla(UGC) gene between the rrs and the rrl genes, whereas another has a tRNAIle(GAU) gene. Phylogenetic analyses suggest that the plastids of Heterokontophyta, Cryptophyta and Haptophyta originated from the subphylum Rhodophytina. The order of the genes in the ribosomal protein cluster of the P. purpureum plastid genome differs from that of other Rhodophyta and Chromalveolata. These results suggest that a large-scale rearrangement occurred in the plastid genome of P. purpureum after its separation from other Rhodophyta.

    Original languageEnglish
    Pages (from-to)389-397
    Number of pages9
    JournalJournal of Plant Research
    Volume127
    Issue number3
    DOIs
    Publication statusPublished - 2014 Jan 1

    Keywords

    • Genome rearrangement
    • Plastid genome
    • Porphyridium purpureum
    • Rhodophyta
    • rRNA operon

    ASJC Scopus subject areas

    • Plant Science

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  • Cite this

    Tajima, N., Sato, S., Maruyama, F., Kurokawa, K., Ohta, H., Tabata, S., Sekine, K., Moriyama, T., & Sato, N. (2014). Analysis of the complete plastid genome of the unicellular red alga Porphyridium purpureum. Journal of Plant Research, 127(3), 389-397. https://doi.org/10.1007/s10265-014-0627-1