Analysis of Sugar Compositions of Glycoproteins Electroblotted onto PVDF Membranes

Koji Muramoto, Hisao Kamiya

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    A method was developed for analysis of sugar compositions of glycoproteins electroblotted onto polyvinylidene difluoride (PVDF) membranes after SDS-polyacrylamide gel electrophoresis. It was based on hydrolysis of glycoproteins on PVDF membranes and precolumn derivatization of liberated neutral sugars, sialic acids and aminosugars, respectively, and isocratic reversed-phase high-performance liquid chromatographies of these derivatives. The presence of sialoglycoproteins in ecbinoderm shell matrixes was demonstrated by this method.

    Original languageEnglish
    Pages (from-to)1121-1126
    Number of pages6
    JournalNIPPON SUISAN GAKKAISHI
    Volume57
    Issue number6
    DOIs
    Publication statusPublished - 1991 Jan 1

    ASJC Scopus subject areas

    • Aquatic Science

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