Analysis of Reducing Sugars in Fish Muscle as Their Chromophoric Hydrazones by High-Performance Liquid and Thin-Layer Chromatography

Koji Muramoto; Rina Goto; Hisao Kamiya

doi:10.2331/suisan.56.967

Analysis of Reducing Sugars in Fish Muscle as Their Chromophoric Hydrazones by High-Performance Liquid and Thin-Layer Chromatography

Koji Muramoto, Rina Goto, Hisao Kamiya

Research output: Contribution to journal › Article

1 Citation (Scopus)

Abstract

A method was developed for the analysis of reducing sugars extracted with 80% aqueous ethanol from fish muscle. It was based on the reaction of the reducing sugars with the chromophoric reagent, 4-N, N-dimethylamino-4'-azobenzene sulfonyl hydrazide, and their separation by reversed-phase high-performance liquid chromatography (HPLC) or high-performance silical gel thin-layer chromatography. The absorbance at 485 nm provided a sensitivity of the picomole level. This method was applied to the reducing sugar analysis of 20 mg of fish muscle. The change of reducing sugar contents in fish muscle of various stages of freshness was compared with that of K values measured by ion-pair reversed-phase HPLC.

Original language	English
Pages (from-to)	967-971
Number of pages	5
Journal	NIPPON SUISAN GAKKAISHI
Volume	56
Issue number	6
DOIs	https://doi.org/10.2331/suisan.56.967
Publication status	Published - 1990 Jan 1

ASJC Scopus subject areas

Aquatic Science

Access to Document

10.2331/suisan.56.967

Fingerprint Dive into the research topics of 'Analysis of Reducing Sugars in Fish Muscle as Their Chromophoric Hydrazones by High-Performance Liquid and Thin-Layer Chromatography'. Together they form a unique fingerprint.

Cite this

Muramoto, K., Goto, R., & Kamiya, H. (1990). Analysis of Reducing Sugars in Fish Muscle as Their Chromophoric Hydrazones by High-Performance Liquid and Thin-Layer Chromatography. NIPPON SUISAN GAKKAISHI, 56(6), 967-971. https://doi.org/10.2331/suisan.56.967

@article{5adae2a5159b480e97201c5d42ed39d7,

title = "Analysis of Reducing Sugars in Fish Muscle as Their Chromophoric Hydrazones by High-Performance Liquid and Thin-Layer Chromatography",

abstract = "A method was developed for the analysis of reducing sugars extracted with 80% aqueous ethanol from fish muscle. It was based on the reaction of the reducing sugars with the chromophoric reagent, 4-N, N-dimethylamino-4'-azobenzene sulfonyl hydrazide, and their separation by reversed-phase high-performance liquid chromatography (HPLC) or high-performance silical gel thin-layer chromatography. The absorbance at 485 nm provided a sensitivity of the picomole level. This method was applied to the reducing sugar analysis of 20 mg of fish muscle. The change of reducing sugar contents in fish muscle of various stages of freshness was compared with that of K values measured by ion-pair reversed-phase HPLC.",

author = "Koji Muramoto and Rina Goto and Hisao Kamiya",

year = "1990",

month = jan,

day = "1",

doi = "10.2331/suisan.56.967",

language = "English",

volume = "56",

pages = "967--971",

journal = "Nippon Suisan Gakkaishi",

issn = "0021-5392",

publisher = "Nihon Suisan Gakkai",

number = "6",

}

TY - JOUR

T1 - Analysis of Reducing Sugars in Fish Muscle as Their Chromophoric Hydrazones by High-Performance Liquid and Thin-Layer Chromatography

AU - Muramoto, Koji

AU - Goto, Rina

AU - Kamiya, Hisao

PY - 1990/1/1

Y1 - 1990/1/1

N2 - A method was developed for the analysis of reducing sugars extracted with 80% aqueous ethanol from fish muscle. It was based on the reaction of the reducing sugars with the chromophoric reagent, 4-N, N-dimethylamino-4'-azobenzene sulfonyl hydrazide, and their separation by reversed-phase high-performance liquid chromatography (HPLC) or high-performance silical gel thin-layer chromatography. The absorbance at 485 nm provided a sensitivity of the picomole level. This method was applied to the reducing sugar analysis of 20 mg of fish muscle. The change of reducing sugar contents in fish muscle of various stages of freshness was compared with that of K values measured by ion-pair reversed-phase HPLC.

AB - A method was developed for the analysis of reducing sugars extracted with 80% aqueous ethanol from fish muscle. It was based on the reaction of the reducing sugars with the chromophoric reagent, 4-N, N-dimethylamino-4'-azobenzene sulfonyl hydrazide, and their separation by reversed-phase high-performance liquid chromatography (HPLC) or high-performance silical gel thin-layer chromatography. The absorbance at 485 nm provided a sensitivity of the picomole level. This method was applied to the reducing sugar analysis of 20 mg of fish muscle. The change of reducing sugar contents in fish muscle of various stages of freshness was compared with that of K values measured by ion-pair reversed-phase HPLC.

UR - http://www.scopus.com/inward/record.url?scp=85008120537&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85008120537&partnerID=8YFLogxK

U2 - 10.2331/suisan.56.967

DO - 10.2331/suisan.56.967

M3 - Article

AN - SCOPUS:85008120537

VL - 56

SP - 967

EP - 971

JO - Nippon Suisan Gakkaishi

JF - Nippon Suisan Gakkaishi

SN - 0021-5392

IS - 6

ER -