TY - JOUR
T1 - Analysis of Reducing Sugars in Fish Muscle as Their Chromophoric Hydrazones by High-Performance Liquid and Thin-Layer Chromatography
AU - Muramoto, Koji
AU - Goto, Rina
AU - Kamiya, Hisao
PY - 1990/1/1
Y1 - 1990/1/1
N2 - A method was developed for the analysis of reducing sugars extracted with 80% aqueous ethanol from fish muscle. It was based on the reaction of the reducing sugars with the chromophoric reagent, 4-N, N-dimethylamino-4'-azobenzene sulfonyl hydrazide, and their separation by reversed-phase high-performance liquid chromatography (HPLC) or high-performance silical gel thin-layer chromatography. The absorbance at 485 nm provided a sensitivity of the picomole level. This method was applied to the reducing sugar analysis of 20 mg of fish muscle. The change of reducing sugar contents in fish muscle of various stages of freshness was compared with that of K values measured by ion-pair reversed-phase HPLC.
AB - A method was developed for the analysis of reducing sugars extracted with 80% aqueous ethanol from fish muscle. It was based on the reaction of the reducing sugars with the chromophoric reagent, 4-N, N-dimethylamino-4'-azobenzene sulfonyl hydrazide, and their separation by reversed-phase high-performance liquid chromatography (HPLC) or high-performance silical gel thin-layer chromatography. The absorbance at 485 nm provided a sensitivity of the picomole level. This method was applied to the reducing sugar analysis of 20 mg of fish muscle. The change of reducing sugar contents in fish muscle of various stages of freshness was compared with that of K values measured by ion-pair reversed-phase HPLC.
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U2 - 10.2331/suisan.56.967
DO - 10.2331/suisan.56.967
M3 - Article
AN - SCOPUS:85008120537
VL - 56
SP - 967
EP - 971
JO - Nippon Suisan Gakkaishi
JF - Nippon Suisan Gakkaishi
SN - 0021-5392
IS - 6
ER -