Analysis of Reducing Sugars in Fish Muscle as Their Chromophoric Hydrazones by High-Performance Liquid and Thin-Layer Chromatography

Koji Muramoto, Rina Goto, Hisao Kamiya

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    A method was developed for the analysis of reducing sugars extracted with 80% aqueous ethanol from fish muscle. It was based on the reaction of the reducing sugars with the chromophoric reagent, 4-N, N-dimethylamino-4'-azobenzene sulfonyl hydrazide, and their separation by reversed-phase high-performance liquid chromatography (HPLC) or high-performance silical gel thin-layer chromatography. The absorbance at 485 nm provided a sensitivity of the picomole level. This method was applied to the reducing sugar analysis of 20 mg of fish muscle. The change of reducing sugar contents in fish muscle of various stages of freshness was compared with that of K values measured by ion-pair reversed-phase HPLC.

    Original languageEnglish
    Pages (from-to)967-971
    Number of pages5
    JournalNIPPON SUISAN GAKKAISHI
    Volume56
    Issue number6
    DOIs
    Publication statusPublished - 1990 Jan 1

    ASJC Scopus subject areas

    • Aquatic Science

    Fingerprint Dive into the research topics of 'Analysis of Reducing Sugars in Fish Muscle as Their Chromophoric Hydrazones by High-Performance Liquid and Thin-Layer Chromatography'. Together they form a unique fingerprint.

  • Cite this