Analysis of PPAR alpha function in human kidney cell line using siRNA.

Keisuke Tachibana, Naohiko Anzai, Chihiro Ueda, Tatsuya Katayama, Takayoshi Kirino, Rika Takahashi, Daisuke Yamasaki, Kenji Ishimoto, Toshiya Tanaka, Takao Hamakubo, Yukihiko Ueda, Hiroyuki Arai, Juro Sakai, Tatsuhiko Kodama, Takefumi Doi

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10 Citations (Scopus)

Abstract

The peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that belong to the nuclear hormone receptor superfamily. PPARalpha is mainly expressed in the liver, kidney, heart and muscle. PPARalpha activates fatty acid catabolism, stimulates gluconeogenesis and ketone body synthesis and is involved in the control of lipoprotein assembly. Although PPARalpha is well characterized in the liver, its physiological function is unknown in the kidney. To investigate the intimate function of PPARalpha in the kidney, we analyzed the target gene expression in human metastatic renal cell carcinoma cell line, Caki-1, using small interfering RNA (siRNA) against PPARalpha and real-time RT-PCR methods. We found that some selected genes (long-chain fatty-acid-CoA ligase (FACL1), carnitine palmitoyltransferase 1A (CPT1A), adipose differentiation-related protein (ADRP) and aquaporin 3 (AQP3)) were down-regulated by PPARalpha siRNA. These results suggest that PPARalpha regulates fatty acid metabolism and body water homeostasis in this cell line.

Original languageEnglish
Pages (from-to)257-258
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number50
Publication statusPublished - 2006
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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  • Cite this

    Tachibana, K., Anzai, N., Ueda, C., Katayama, T., Kirino, T., Takahashi, R., Yamasaki, D., Ishimoto, K., Tanaka, T., Hamakubo, T., Ueda, Y., Arai, H., Sakai, J., Kodama, T., & Doi, T. (2006). Analysis of PPAR alpha function in human kidney cell line using siRNA. Nucleic acids symposium series (2004), (50), 257-258.