@article{cac4b64ea6b141eb9271cdf472af063a,
title = "Analysis of oscillatory rocking curve by dynamical diffraction in protein crystals",
abstract = "High-quality protein crystals meant for structural analysis by X-ray diffraction have been grown by various methods. The observation of dynamical diffraction in protein crystals is an interesting topic because dynamical diffraction generally occurs in perfect crystals such as Si crystals. However, to our knowledge, there is no report yet on protein crystals showing clear dynamical diffraction. We wonder whether the perfection of protein crystals might still be low compared with that of high-quality Si crystals. Here, we present observations of the oscillatory profile of rocking curves for protein crystals such as glucose isomerase crystals. The oscillatory profiles are in good agreement with those predicted by the dynamical theory of diffraction. We demonstrate that dynamical diffraction occurs even in protein crystals. This suggests the possibility of the use of dynamical diffraction for the determination of the structure and charge density of proteins.",
keywords = "Dynamical diffraction, Glucose isomerase, Protein crystal, Rocking curve, X-ray topography",
author = "Ryo Suzuki and Haruhiko Koizumi and Keiichi Hirano and Takashi Kumasaka and Kenichi Kojima and Masaru Tachibana",
note = "Funding Information: ACKNOWLEDGMENTS. We thank Dr. H. Sugiyama of the High Energy Accelerator Research Organization (KEK) and Dr. S. Baba and Dr. N. Mizuno of the Japan Synchrotron Radiation Research Institute (JASRI) for their help with the synchrotron radiation X-ray experiments. We also thank Dr. H. Murata of Yokohama City University for his critical comments on the data analysis. Rocking curve measurements with X-ray topography were performed in BL20B at the Photon Factory of KEK under the approval of the Program Advisory Committee (Proposals 2014G601, 2015G142, and 2017G087). The rocking curve measurements with X-ray topography were also carried out in BL38B1 at SPring-8 with the approval of the JASRI (Proposals 2014A1850, 2014B1965, 2015A1994, 2015B1979, and 2017A2562). This work was partly supported by Japan Society for the Promotion of Science (JSPS) KAKENHI Grants-in-Aid for Scientific Research (25420694 and 16K06708) and Accelerated Innovation Research Initiative Turning Top Science and Ideas into High-Impact Values (JST-ACCEL) (JPMJAC1304). Funding Information: . We thank Dr. H. Sugiyama of the High Energy Accelerator Research Organization (KEK) and Dr. S. Baba and Dr. N. Mizuno of the Japan Synchrotron Radiation Research Institute (JASRI) for their help with the synchrotron radiation X-ray experiments. We also thank Dr. H. Murata of Yokohama City University for his critical comments on the data analysis. Rocking curve measurements with X-ray topography were performed in BL20B at the Photon Factory of KEK under the approval of the Program Advisory Committee (Proposals 2014G601, 2015G142, and 2017G087). The rocking curve measurements with X-ray topography were also carried out in BL38B1 at SPring-8 with the approval of the JASRI (Proposals 2014A1850, 2014B1965, 2015A1994, 2015B1979, and 2017A2562). This work was partly supported by Japan Society for the Promotion of Science (JSPS) KAKENHI Grants-in-Aid for Scientific Research (25420694 and 16K06708) and Accelerated Innovation Research Initiative Turning Top Science and Ideas into High-Impact Values (JST-ACCEL) (JPMJAC1304). Publisher Copyright: {\textcopyright} 2018 National Academy of Sciences. All right reserved.",
year = "2018",
month = apr,
day = "3",
doi = "10.1073/pnas.1720098115",
language = "English",
volume = "115",
pages = "3634--3639",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "14",
}