Analysis of oscillatory rocking curve by dynamical diffraction in protein crystals

Ryo Suzuki, Haruhiko Koizumi, Keiichi Hirano, Takashi Kumasaka, Kenichi Kojima, Masaru Tachibana

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

High-quality protein crystals meant for structural analysis by X-ray diffraction have been grown by various methods. The observation of dynamical diffraction in protein crystals is an interesting topic because dynamical diffraction generally occurs in perfect crystals such as Si crystals. However, to our knowledge, there is no report yet on protein crystals showing clear dynamical diffraction. We wonder whether the perfection of protein crystals might still be low compared with that of high-quality Si crystals. Here, we present observations of the oscillatory profile of rocking curves for protein crystals such as glucose isomerase crystals. The oscillatory profiles are in good agreement with those predicted by the dynamical theory of diffraction. We demonstrate that dynamical diffraction occurs even in protein crystals. This suggests the possibility of the use of dynamical diffraction for the determination of the structure and charge density of proteins.

Original languageEnglish
Pages (from-to)3634-3639
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume115
Issue number14
DOIs
Publication statusPublished - 2018 Apr 3

Keywords

  • Dynamical diffraction
  • Glucose isomerase
  • Protein crystal
  • Rocking curve
  • X-ray topography

ASJC Scopus subject areas

  • General

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