Analysis of mutations in the thymidine kinase gene of varicella zoster virus associated with resistance to 5-iodo-2′-deoxyuridine and 5-bromo-2′-deoxyuridine

Eiichi Kodama, Shuichi Mori, Shiro Shigeta

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

We have analyzed mutations in the thymidine kinase (TK) gene of varicella zoster virus (VZV) which showed resistance to 5-iodo-2′-deoxyuridine (IDU) and 5-bromo-2′-deoxyuridine (BrDU). Through sequencing of the TK gene, we found three amino acids were exchanged (41 Asn → Ser, 266 Cys → Ile, 288 Ser → Leu). These mutations were not located at either the nucleoside- or the ATP-binding site. This result suggests that the resistance to IDU and BrDU in this particular strain is due to the change in conformation of TK rather than the replacement of amino acids in the binding sites.

Original languageEnglish
Pages (from-to)165-170
Number of pages6
JournalAntiviral Research
Volume27
Issue number1-2
DOIs
Publication statusPublished - 1995 May
Externally publishedYes

Keywords

  • Sequence analysis
  • Thymidine kinase
  • Varicella zoster virus

ASJC Scopus subject areas

  • Pharmacology
  • Virology

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