TY - JOUR
T1 - Analysis of gene expression profiles in alveolar epithelial type II-like cells differentiated from human alveolar epithelial progenitor cells
AU - Fujino, Naoya
AU - Ota, Chiharu
AU - Suzuki, Takaya
AU - Suzuki, Satoshi
AU - Hegab, Ahmed E.
AU - Yamada, Mitsuhiro
AU - Takahashi, Toru
AU - He, Mei
AU - Kondo, Takashi
AU - Kato, Hidemasa
AU - Yamaya, Mutsuo
AU - Kubo, Hiroshi
N1 - Funding Information:
We are grateful to Professor Ryouichi Nagatomi (Department of Biomedical Engineering, Tohoku University Graduate School of Biomedical Engineering, Tokyo, Japan) for his advice on this project. We would also like to thank the Biomedical Research Core of Tohoku University Graduate School of Medicine, Tokyo, Japan, for their technical support. This work was supported by a Grant from the Japan Society for the Promotion of Science (No. 22390163 ) to HK.
PY - 2012/9
Y1 - 2012/9
N2 - Background: Damage to lung epithelial cells through chronic injury and abnormal repair and remodeling lead to lung destruction and fibrosis. We isolated lung progenitor cells that could potentially contribute to lung diseases. The progenitor cells can differentiate into alveolar type II (ATII)-like cells in vitro, and are increased in number and localized within the region of alveolar epithelial cell proliferation that is involved in the reparative response to injury. However, global gene expression patterns in the ATII-like cells derived from the progenitor cells and in mature ATII cells isolated from lung tissue have not yet been evaluated. Methods: We performed gene expression array and directly compared the gene expression patterns in ATII-like cells derived from the progenitor cells with those in mature ATII cells isolated from human lung tissues. Results: ATII-like cells and mature ATII cells expressed certain common genes, such as CEPBD and FOXP1, which determine the phenotypes of ATII cells. However, many genes were differentially expressed between the 2 cell types. As compared to mature ATII cells, ATII-like cells showed decreased expression of the genes associated with surfactant protein production and epithelial phenotypes. Pathway analysis indicated changes in several pathways, including those involved in epithelial-to-mesenchymal transition and receptor tyrosine kinase signaling, which could contribute to the observed differences in gene expression patterns. Conclusions: In this study, we identified genes commonly or differentially expressed by ATII-like cells differentiated from progenitor cells and mature ATII cells isolated from human lung tissues.
AB - Background: Damage to lung epithelial cells through chronic injury and abnormal repair and remodeling lead to lung destruction and fibrosis. We isolated lung progenitor cells that could potentially contribute to lung diseases. The progenitor cells can differentiate into alveolar type II (ATII)-like cells in vitro, and are increased in number and localized within the region of alveolar epithelial cell proliferation that is involved in the reparative response to injury. However, global gene expression patterns in the ATII-like cells derived from the progenitor cells and in mature ATII cells isolated from lung tissue have not yet been evaluated. Methods: We performed gene expression array and directly compared the gene expression patterns in ATII-like cells derived from the progenitor cells with those in mature ATII cells isolated from human lung tissues. Results: ATII-like cells and mature ATII cells expressed certain common genes, such as CEPBD and FOXP1, which determine the phenotypes of ATII cells. However, many genes were differentially expressed between the 2 cell types. As compared to mature ATII cells, ATII-like cells showed decreased expression of the genes associated with surfactant protein production and epithelial phenotypes. Pathway analysis indicated changes in several pathways, including those involved in epithelial-to-mesenchymal transition and receptor tyrosine kinase signaling, which could contribute to the observed differences in gene expression patterns. Conclusions: In this study, we identified genes commonly or differentially expressed by ATII-like cells differentiated from progenitor cells and mature ATII cells isolated from human lung tissues.
KW - Alveolar epithelial type II cell
KW - Epithelial-to-mesenchymal transition
KW - Lung progenitor cell
KW - Microarray
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U2 - 10.1016/j.resinv.2012.07.002
DO - 10.1016/j.resinv.2012.07.002
M3 - Article
C2 - 23021770
AN - SCOPUS:84875818055
VL - 50
SP - 110
EP - 116
JO - Respiratory Investigation
JF - Respiratory Investigation
SN - 2212-5345
IS - 3
ER -