Analysis of gene expression during the neural and primitive-endodermal differentiation of P19 cells using DNA microarray

Yoshiyuki Sakamoto, Shuichi Yoshihara, Takayasu Kobayashi, Shinri Tamura, Mutsuo Sasaki

Research output: Contribution to journalArticlepeer-review

Abstract

P19 cells resemble the inner cell mass of early embryos and have an ability to differentiate into three germ layers depending on the culture condition. Treatment of P19 cells with higher concentrations (> 10-6 M) of retinoic acid leads to differentiation to neurons and glias, while the treatment of P19 cells with low concentrations (∼10-8 M) of retinoic acid results in differentiation into primitive endoderm-like cells. In this study, we profiled global gene expression patterns during the neural and endodermal differentiation of P19 cells using a cDNA microarray. We chose Snk and SKAP55R whose expression was found to be enhanced in the early phase of the neural differentiation of P19 cells on a cDNA microarray. The results of Northern analysis showed that the expression of Snk was found to be enhanced as P19 cells differentiated to either neurons or endodermal-like cells. The expression of SKAP55R was up-regulated in the terminal stage of the primitive endodermal differentiation, while in the course of the neural differentiation SKAP55R was up-regulated in the early stage and down-regulated in the terminal stage. These results suggest that both Snk and SKAP55R participate in the regulation of neural and endodermal differentiation but in different modes and timing.

Original languageEnglish
Pages (from-to)117-135
Number of pages19
JournalHirosaki Medical Journal
Volume54
Issue number3-4
Publication statusPublished - 2003

Keywords

  • P19 embryonic carcinoma cells
  • SKAP55R
  • Signal transductions
  • Snk
  • cDNA microarray

ASJC Scopus subject areas

  • Medicine(all)

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