Analysis of gene amplification in human tumor cells by in-gel DNA renaturation assay.

Manabu Fukumoto, H. Tashiro, I. B. Roninson

Research output: Contribution to journalArticlepeer-review

Abstract

The incidence of gene amplification in leukemia/lymphoma is significantly lower than that in solid tumors. For understanding the biological characteristics of leukemia/lymphoma, it is necessary to clarify whether the incidence of gene amplification is actually lower or gene amplification specific to hemopoietic malignancies has not yet been found. We describe a new version of in-gel DNA renaturation technique for general screening of gene amplification that allows detection and cloning of amplified genes of unknown nature as few as 7- to 8-fold amplification.

Original languageEnglish
Pages (from-to)1463-1470
Number of pages8
JournalActa Haematologica Japonica
Volume52
Issue number8
Publication statusPublished - 1989 Dec 1

ASJC Scopus subject areas

  • Hematology

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