Analysis of functional domains of a GC box-binding protein, BTEB1

Akira Kobayashi, Kazuhiro Sogawa, Hiroaki Imataka, Yoshiaki Fujii-kuriyama

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    31 Citations (Scopus)

    Abstract

    BTEB is a small GC box-binding protein containing three contiguous zinc finger structures in the C-terminal region of the molecule, and activates or represses the transcription of genes with the GC box sequence in the promoter, depending on the repetitiveness of the GC box sequence [Imataka et al. (1992) EMBO J. 11, 3663-3671]. We have analyzed functional domains of BTEB in a transient expression system using Y-l cells (a mouse adrenal cortex cell line). BTEB contained two regions responsible for the transcriptional activation. These regions showed a sequence similarity to each other and appeared to enhance the transcription independently. The two sequences were rich in hydrophobic amino acids and showed no similarity to the sequences of transactivation domains so far elucidated. The DNA-binding properties of BTEB were also analyzed by means of the gel mobility shift assay. Three contiguous zinc finger motifs were needed for the binding activity. Furthermore, it was revealed that a short basic region immediately N-terminal to the zinc finger motifs was required for the DNA binding.

    Original languageEnglish
    Pages (from-to)91-95
    Number of pages5
    JournalJournal of biochemistry
    Volume117
    Issue number1
    DOIs
    Publication statusPublished - 1995 Jan

    Keywords

    • DNA-binding protein
    • Transcription factor
    • Zinc finger

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

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